Fig. 3

NOSTRIN down-regulates proteins associated with epithelial-mesenchymal transition (EMT) in HCT116 cells. Western-blot analysis of A NOSTRIN and B-F the differentially regulated EMT marker genes in control and NOSTRIN over-expressing HCT116 cells. Western blots are grouped based on functional annotation. B Transcription factors: SMAD2, SNAI2 and STAT3. C Proteins involved in cell growth and proliferation, ILK1, RGS2 and OCLN D Proteins involved in cell migration and motility, JAG1, MSN and CALD1 E Proteins associated with extracellular matrix and cell adhesion molecules COL3A1, ITGα5, F11R, Versican (VCAN) and E-cadherin (CDH1). F Cytoskeleton regulating proteins, Vimentin (VIM) and desmoplakin (DSP). Quantification of the proteins relative to GAPDH using three biological replicates using NIH ImageJ software has been shown in adjacent bar graphs. Error bars represent standard error of mean from three independent biological replicates. *p < 0.05, **p < 0.01. Full length blots are shown in Fig. S3