Fig. 7

Characterize the co-expression and correlation of TNFRSF4 with immune-related genes in EC TAMs. A, B, C Correlation of TNFRSF4 with CD4, CD8A, and FOXP3 analyzed from EC-TCGA database. D Representative IHC staining for TNFRSF4, CD4, CD8, and FOXP3 in EC and adjacent normal tissue. × 200 magnification (scale bar = 100 μm). E, F Violin plots visualizing the quantification of TNFRSF4, CD4, and CD8 in EC compared with adjacent normal tissue, 36 cases were included in paired comparison, and 85 ECs and 36 normal tissue were included in unpaired comparison. G, H ROC curves analyzing the diagnostic performance of TNFRSF4, CD4, CD8A, and FOXP3 for EC patients in the TCGA and clinical specimens by pROC package. I Representative endometrial carcinoma stained with five markers (CK, CD4, CD8, FOXP3, and TNFRSF4). Individual markers were displayed on the left, and merged images were displayed on the right. Scale bar: 100 μm