Fig. 5
From: The identification and validation of EphA7 hypermethylation, a novel biomarker, in cervical cancer
Validation of the potential value of EphA7 methylation for the diagnosis of CC. a The methylation-positive rate was 0% (0/25) in normal tissues, 45.83% (11/24) in CIN II/III, and 100% (8/8) in cancer tissues via MSP. Leu: Leukocyte DNA from healthy women was used as a negative control of methylation. iv: In vitro methylated leukocyte DNA was used as a positive control. Methylation positive results: the methylated bands were heavier than the unmethylated bands. Methylation negative results: the methylated bands were lighter than the unmethylated bands. Full-length gel pictures are presented in Figure. s3. b QMSP showed that the methylation level of EphA7 increased with the severity of cervical lesions (H = 27.311, P < 0.001)