Fig. 3
From: The identification and validation of EphA7 hypermethylation, a novel biomarker, in cervical cancer
Targeted DNA demethylation of the EphA7 promoter using the dCas9-Tet1 system in CC cell lines. The expression of EphA7 was upregulated at various levels in CaSki (a) and SiHa (d) cells transfected with combinations of dCas9-Tet1 with sgRNAs compared with the control(Tet1m, Tet1m was Tet1 mutations, n = 3). Pyrosequencing showed that the EphA7 promoter methylation d was lower in the dCas9-Tet1 group compared with Tet1m in CaSki (b) and SiHa (e) (n = 3).The average methylation level in the promoter region of EphA7 in the sgRNA1 group respectively decrease by 19.10% and 17.23% in CaSki (c) and SiHa (f) cells, respectively, compared with the control (n = 3)