Fig. 5

SKOV3-M cells display differential gene expression and invasive capacity than its parental cell lines. a Heat maps of genes expression in SKOV3-M, SKOV3-Green, and SKOV3-Red. Colors from yellow to blue indicate downregulated cellular genes; colors from yellow to red indicate upregulated cellular genes. b MMP-2 activity in the culture supernatant of SKOV3-M, SKOV3-Green, and SKOV3-Red. White bands were quantified using the ImageJ software, and data were normalized by protein concentration and expressed as the fold change relative to the control. The original gel of the three experiments is shown in supplementary Fig. 5. c The invasive capacity of SKOV3-Red, SKOV3-Green, and SKOV3-M cells was assessed by the Boyden chamber assay. Cell viability was assessed by MTT assay. Results are expressed as invasion relative to cell viability ± SEM. AU: arbitrary unit, * p < 0.05, ** p < 0.01, ***p < 0.001