Fig. 1

Laminin promoted cell proliferation and migration in bladder cancer. A, immunohistochemical staining of laminin in tumor tissues from NMIBC and MIBC patients. The expression of laminin was quantified in each group (n = 15). The scale bar was 50 μm. B, cell proliferation of Biu-87 and MB49 cells treated with PBS or laminin (2 μg/ml). C, the relative migrating cells of Biu-87 and MB49 cells treated with PBS or laminin (2 μg/ml). D, cell proliferation of Biu-87 and MB49 cells pre-cultured on dish, 2D collagen and 2D laminin/collagen gels. E, the relative migrating cells of Biu-87 and MB49 cells pre-cultured on dish, 2D collagen and 2D laminin/collagen gels. F, cell proliferation of Biu-87 cells pre-cultured on dish, 2D fibrin and 2D laminin/fibrin gels. G, the relative migrating cells of Biu-87 pre-cultured on dish, 2D fibrin and 2D laminin/fibrin gels. H, survival analysis of bladder cancer patients with high/low LAMC1 expression using TCGA database (n = 405)