Fig. 6

RNF38 interacts with ACTN4 and induces ubiquitylation and degradation of ACTN4. A ACTN4 expression in normal nasopharyngeal tissues and NPC tissues by PCR (Left column). Correlation of ACTN4 and RNF38 expression in nasopharyngeal carcinoma samples (Right column). B Coimmunoprecipitation detected the interaction of RNF38 and ACTN4 in SUNE-1 cells. C CHX chase assay was used to examine the half-life of ACTN4 in SUNE-1 cells treated with CHX for 0, 2 and 4 hours. D Western blotting was performed to observe the levels of RNF38, ACTN4, p-p65 and p-Erk1/2 in SUNE-1 cells treated with DMSO or MG132. E Immunoblotting using an anti-ubiquitin antibody was performed to examine the ubiquitination levels of ACTN4. F, Clone formation assays, proliferation assays (G), cell migration and invasion assays (H) in RNF38 overexpressing SUNE-1 cells with or without upregulation of ACTN4. I, Levels of p-p65 and p-Erk1/2 in overexpressing RNF38 SUNE-1 cells with or without upregulation of ATCN4