Fig. 2

RNF38 inhibits the proliferation of NPC cells in vitro and in vivo. A Overexpression of RNF38 (RNF38), downregulation of RNF38 (in-RNF38), and the related normal controls (NC or in-NC) in NPC cells verified by Western blot and RT-PCR. B-C Cell proliferation assays and clone formation assays. D-E Subcutaneous injection of SUNE-1 cells stably overexpressing or downregulating RNF38 in the xenograft model (n = 6). Xenograft tumor volume and tumor weight were analysed. F Representative images and quantification of the expression of Ki67 in xenograft tumors. Data are presented as the mean ± SEM; P values were calculated using Student’s t-test. *p < 0.05, ** p < 0.01, *** p < 0.001