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Table 1 Primer sequences for RT-qPCR

From: KDM3A-mediated SP1 activates PFKFB4 transcription to promote aerobic glycolysis in osteosarcoma and augment tumor development

Gene

Forward primer (5′-3′)

Reverse primer (5′-3′)

KDM3A

GCCAACATTGGAGACCACTTCTG

CTCGAACACCTTTGACAGCTCG

SP1

ACGCTTCACACGTTCGGATGAG

TGACAGGTGGTCACTCCTCATG

PFKFB4

GATCCTGAGGTCATAGCTGCCA

CTATCCAGGTCCTCATCTAGCG

SIN3A

CAGAATGACACCAAGGTCCTGAG

CATACGCAAGTGAGAGGTGTGG

GAPDH

GTCTCCTCTGACTTCAACAGCG

ACCACCCTGTTGCTGTAGCCAA

  1. RT-qPCR Reverse transcription quantitative polymerase chain reaction, KDM3A Lysine-specific histone demethylase 3A, SP1 Sp1 transcription factor, PFKFB4 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4, SIN3A SIN3 transcription regulator family member A, GAPDH glyceraldehyde-3-phosphate dehydrogenase
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BMC Cancer

ISSN: 1471-2407

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