Fig. 2

Creation of disease models of pancreatic cancer using PDOs derived from primary and disseminated patient tumor samples. A. Subcutaneous transplantation of PDOs and histological analysis of the formed tumors. Schematic representation of transplantation experiments (left top). Shown are selected examples of tumors of PDOs (right top: HE-stained). Scale bar, 100 μm. Subcutaneous tumorigenicity test of PDOs (bottom). Number of mice with tumors per total number of PDO-transplanted mice at 11 weeks after transplantation. NT, not tested. B. Schematic illustration of intra-peritoneal injection of PDOs into nude mice (top). Histochemical analysis of formed disseminated tumor nodule (left: HE-stained, right: CD44 IHC). Scale bar, 200 μm. C. Intraperitoneal tumor nodule formation in mice transplanted with PDOs. Three different PDOs were injected into nude mice (N = 5), and tumor nodule formation was evaluated