Fig. 5

DAB2IP regulates the apoptosis of CRC cells through HSP90AA1/SRP9/ASK1/JNK axis. a Apoptosis detection after SRP9 silencing by flow cytometry in CRC cell lines. Apoptosis was analyzed by flow cytometry. *P < 0.05, ** P < 0.01, ***P < 0.001, ****P < 0.0001. The data were expressed as mean ± SD, n = 3. b Apoptosis of CRC cells was regulated by DAB2IP/HSP90AA1/SRP9 signal pathway. Apoptosis was analyzed by flow cytometry. *P < 0.05, ** P < 0.01, ***P < 0.001, ****P < 0.0001. The data were expressed as mean ± SD, n = 3. c d Overexpression of DAB2IP promoted apoptosis or knockdown of DAB2IP decreased apoptosis through the ASK1/JNK pathway. e f Knockdown of HSP90AA1 increased apoptosis or activation of HSP90AA1 by TZ inhibited apoptosis through the ASK1/JNK pathway. g Knockdown of SRP9 increased apoptosis through the ASK1/JNK pathway. h Loss of DAB2IP impeded apoptosis through HSP90AA1/SRP9/ASK1/JNK axis in CRC cells