Fig. 5
Disulfiram/copper complex reduces LGR5-positive cervical cancer cells. a-b The LGR5-overexpressing cervical cancer cells and the control cells were treated with 0.1 μM disulfiram plus 0.01 μM CuCl2 (DSF/Cu) or 10 μM DDP or DSF/Cu plus 10 μM DDP (DSF/Cu/DDP) for 24 h and 48 h, after which their cell viabilities were determined by the MTT assay. c-d The percentage of LGR5-positive cells in the cervical cancer cell lines was analyzed by flow cytometry after exposure to different drugs for 3 days and following culture in ordinary culture medium for 2 weeks. e-f Graphical representation of the statistical analysis of the percentage of LGR5-positive cells in different groups. Data represent mean ± S.D. of triplicate experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001