Fig. 4

LMOD1 enhanced the migration ability of gastric cancer cells through the FAK-Akt/mTOR pathway. A The expression of LMOD1 in cell lines was measured using western blots. B The expression of LMOD1 in cell lines was measured by RT-qPCR. C-F A GSEA analysis of LMOD1 enriched pathways in TCGA and GSE62254 databases. G, H Western blotting was used to measure protein markers of the FAK-Akt/mTOR pathway in Control or LMOD1- silenced SGC-7901 and MGC-803 cell lines. I Western blotting was used to measure protein markers of the FAK-Akt/mTOR pathway in the Control or LMOD1-overexpressed HGC-27 cell line. J, K The migration ability of cells was detected using a scratch test. M, N The migration ability of cells was measured using a transwell test. The membrane was cut out due to the different secondary antibodies incubated. *, P < 0.05, **, P < 0.01, ***, P < 0.001, ****, P < 0.0001