Fig. 3

Silencing BIRC5 depressed IFN-γ-induced aggravation of PC cell processes. PC cells were transfected with BIRC shRNAb lentiviral vector before IFN-γ treatment (20 ng/mL, 24 h). (A and B) CCK-8 assay detected cell viability. (C, D and E) RT-qPCR and western blot assays were performed to measure the expression of BIRC5. In A and C, * p < 0.05 and ** p < 0.01 versus 2.5 ng/mL group, ^# p < 0.05 and ^## p < 0.01 versus 5 ng/mL group, ^$$ p < 0.01 versus 10 ng/mL; in B, D and E, ** p < 0.01 versus 0 h, ^# p < 0.05 and ^## p < 0.01 versus 6 h, ^$$ p < 0.01 versus 12 h and 24 h respectively. (F, G and H) Cell viability, migration and invasion were evaluated using CCK-8, wound healing and Transwell methods. (I) The expression of EMT proteins were measured by western blot assay. The columns were presented as the mean ± SEM (n ≥ 3). ^* p < 0.05 and ^** p < 0.01