Fig. 2From: BIRC5 regulates inflammatory tumor microenvironment-induced aggravation of penile cancer development in vitro and in vivoSilencing BIRC5 inhibited cell viability, migration, invasion of PC cells. (A) Expression of BIRC5 was detected using RT-qPCR. PC cells were transfected with shRNA lentiviral vectors. (B and C) The silencing efficiency of BIRC5 shRNAs was confirmed by RT-qPCR and western blot. (D) Cell viability, migration and invasion were studied by CCK-8, wound healing and Transwell assays. (G) Western blotting presented the expressions of target EMT proteins. The columns were presented as the mean ± SEM (n ≥ 3). * and ** stood for p < 0.05 and p < 0.01Back to article page