Fig. 3
From: DNA methylation of miR-138 regulates cell proliferation and EMT in cervical cancer by targeting EZH2
Silencing of enhancer of zeste homolog 2 (EZH2) mimics the function of miR-138 overexpression in regulating cervical cancer cell proliferation, migration, and invasion. a Western blot analysis of EZH2 and E-cadherin expression in SiHa and C33A cells transfected with EZH2 or control siRNA. β-actin served as a loading control. b-e Analyses of cell proliferation (b), apoptosis (c), invasion (d), and migration (e) in SiHa and C33A cells transfected with EZH2 siRNA, miR-138 mimic, or negative-control miRNA (NC). f Western blot analysis of EZH2 expression in SiHa and C33A cells co-transfected with siEZH2 and/or miR-138 inhibitor. g Transwell assay after miR-138 inhibitor and siEZH2 co-transfection