Fig. 1

Workflow of this study. All protein biomarker studies on this cohort were based on 386 patients with stage II and III colon cancer. After primary resection, and without disturbing clinical workflows (i.e. diagnostics and the decision of whether or not adjuvant chemotherapy (ACT) was administered), clinical data and tumour tissues were obtained. These tissues were previously analysed for 28 promising biomarkers. These results and routine clinicopathological parameters were included in this CART analysis, with separate analysis for patients not treated and treated with ACT. For each treatment group the classifier was subsequently associated with disease-free survival