Fig. 7

Polarization of THP1 cells and co-culture experiment. A Schematic model co-culture system. B The morphological differences in THP1, M0, and M2 cells under a light field. C Comparison of the MHCC-97H migration using transwell compartments. D Detect M0 and M2 macrophage markers by qPCR to determine the polarization state of macrophages. E–G The expression of IRGs signature genes in the co-culture group and three normal HCC cell lines were detected by qPCR. * P < 0.05, **P < 0.01, *** P < 0.001