Fig. 6
From: Downregulation of KRAB zinc finger proteins in 5-fluorouracil resistant colorectal cancer cells
Quantitative RT-PCR assays on KRAB-ZFP proteins in sensitive and resistant cell lines. Total RNA was extracted from sensitive and resistant DLD-1, HCT-116 and HT-29 cell lines, and qRT-PCR was performed to quantify the relative amount of RNA using primers specific for the indicated genes. The cell lines indicated in red for each of the KRAB-ZFPs are the cell lines displaying downregulation of the mRNA as measured by RNAseq. MRLP19, PUM1 and YWHAZ were used as controls. Error bars indicate standard deviation. Asterisks represent significant P values (two-tailed Student’s t test) comparing the means between samples and their respective controls. *P < 0.05, **P < 0.01 and ***P < 0.001