Fig. 4

αM shows an inhibitory effect on CSCs in an in vivo xenograft mouse model. A Schematic representation of the experimental design. B Body weight of mice was not significantly different between the control group and the other groups during the experiment (Student’s t test). C Tumor volume in the HT29 xenograft mice treated with each agent. The tumor volume of the control group was larger than that in the αM group. Tumor volume of the 5-FU + αM group was significantly larger than that in the 5-FU only group. D Tumors from each group were weighed immediately after resection. The tumor weight in the 5-FU + αM group was significantly lower than that in the 5-FU group. E CD44/CD133 double-positive tumors in the αM group were significantly fewer than those in the αM group. These proportions were significantly lower in the 5-FU + αM group than in the 5-FU group. All Data is N = 5. Data are expressed as mean ± SEM values; ^*P < 0.05, ^**P < 0.01, ^***P < 0.001