Fig. 8

Effect of TIM-1 overexpression on proteins related to the EMT- and PI3K/AKT/mTOR signaling pathway in HeLa and SiHa cells. A, B Western blotting and quantification analysis of the EMT-associated proteins E-cadherin, N-cadherin, vimentin, Snail, MMP-2, and VEGF. C, D Western blotting and quantification analysis of proteins related to the PI3K/AKT/mTOR signaling pathway PI3K, p110α, AKT, p-AKT, and mTOR. Data are shown as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the MOCK and NC groups. TIM-1, T-cell immunoglobulin mucin-1; EMT, epithelial-mesenchymal transition; MMP-2, matrix metalloproteinase 2; VEGF, vascular endothelial growth factor; MOCK, blank control; NC, negative control