Fig. 7
From: Innovative mouse models for the tumor suppressor activity of Protocadherin-10 isoforms
Detection of GFAP protein and GFAP-Cre activity in mouse tissues and tumors. Genotypes of mice analyzed are given on top of the corresponding panels. A,B Immunostaining of pinnal tissues with anti-GFAP antibody. In normal pinnae (A), only perichondrial stem cells (PSC) are positive. In case of pinnal tumors (B), also tumor cells surrounding the elastic cartilage may be positive, but this is often not the case. Scale bars: 50 μm. C,D GFAP-Cre activity in GFAP-Cretg/+;Rosa26tg/+ mice, as detected by LacZ staining. C Positive staining of the granular layer of the cerebellum (young P6 mouse). Sagittal section obtained after whole-mount LacZ staining of the brain. D Positive whole-mount staining at the basis of the pinna (P35 mouse). The broken line indicates the section plane, yielding sections shown in E and F. Scale bars: 100 μm. LacZ-positive cells were seen at the location of PSC. G Strong LacZ activity in a pinnal tumor demonstrates previous GFAP-Cre activity. The arrow points at positivity in ‘normal’ elastic cartilage. H Strong LacZ activity in a cell line derived from a tumor, similar to this depicted in G. Scale bar: 50 μm