Fig. 2
From: Innovative mouse models for the tumor suppressor activity of Protocadherin-10 isoforms
Generation of recombinant mice with floxed Pcdh10 genes. A Targeting strategy to generate Pcdh10allfl/fl mice. loxP sites (black arrowheads) were inserted downstream and upstream of exon 1. A neo-resistance cassette (neo) flanked by FRT sites was inserted downstream of exon 1 for selection of correctly targeted embryonic stem (ES) cells. An HSV-tk cassette was cloned into the targeting vector for negative selection of ES cells with random integration of the targeting vector. B Targeting strategy to generate Pcdh10longfl/fl mice. loxP sites were inserted upstream of exon 2 and downstream of exon 3, respectively. A neo cassette flanked by FRT sites was inserted upstream of exon 2 for selection of correctly targeted ES cells. A and B 5′ and 3′ probes and restriction enzyme sites for Southern blot analysis of ES cells are indicated and expected fragments are shown in grey (Results in Additional file 4: Fig. S3A). Colored arrows indicate the position of genotyping primers used to check the 5′ (red or green arrows) and 3′ (purple and blue arrows) recombined regions (Additional file 4: Fig. S3B). Black rectangles, exons 1–4; CM1 and CM2, conserved motifs