Fig. 2
Expression of RAB3B and sphere formation ability of SK-HEP-1 derivative cells. a, RAB3B mRNA levels in SK-HEP-1 derivative cells. RAB3B mRNA levels were quantified using qRT-PCR, and the values were subsequently normalized as the mean ratio of the value from reference cells (SK-HEP-1 cells cultured in control medium). *P < 0.05 with Tukey–Kramer multiple comparison test. b, Western blot analysis of whole-cell lysates. The upper and lower immunoblottings were generated from the same gel, and the blots were separated around the indicated sizes and then reacted with each antibody, respectively. VCP was used as the loading control. c–e, Sphere cells derived from SK-HEP-1 (c), SK-HEP-1 harboring genome edited RAB3B (d), and the RAB3B edited SK-HEP-1 harboring a RAB3B expressing vector, pRAB3B (e)