Fig. 2

Semiquantitative and quantitative evaluation of tumor associated macrophages (TAMs). A–C Representative cases of CD163⁺ TAMs, A high: zonal dense infiltration (arrows), B intermediate: focal nested infiltration (arrows), C low: entirely sparse infiltration, × 40. D, E Example case of quantitative evaluation of CD163⁺ TAMs using a BZ-X-700 microscope (Keyence), D original photo of immunohistochemical staining for CD163, E \(\mathrm{CD}163\ \mathrm{positive}\ \mathrm{TAMs}\ \mathrm{area}\ \mathrm{ratio}=\frac{\mathrm{CD}163\ \mathrm{positive}\ \mathrm{TAMs}\ \mathrm{area}}{\mathrm{background}\ \mathrm{stroma}\ \mathrm{area}}=\frac{\mathrm{blue}\ \mathrm{area}}{\mathrm{background}-\mathrm{black}\ \left(\mathrm{tumor}\right)\ \mathrm{area}}\)