Fig. 5

CALML3-AS1 acts as a suppressor via regulation of miR-20a-5p/RMB38 axis. A. Relative expression of RMB38 in BCPAP and K1 cells with RBM38 silenced (si-NC, si-RBM38#1, si-RBM38#2). B. Relative expression levels of RBM38 with CALML3-AS1-OE, or both CALML3-AS1 and miR-20a-5p, both hsa_circ_0000069-OE and RBM38 knockdown in BCPAP and K1 cells were measured using qRT-PCR. C. The expression of RMB38 in BCPAP and K1 cells with si-NC, CALML3-AS1 OE, CALML3-AS1 OE + miR-20a-5p mimics and CALML3-AS1 OE + si-RBM38#1 was detected by western blot assay. D. Pearson correlation analysis between CALML3-AS1 and RMB38 expressions in 52 cases of PTC tissues. E. F. CCK-8 and colony formation assays were used to measure the proliferation ability of BCPAP and K1 cells with the above four groups. G. H. Transwell assays were used to determine the effects of four groups on PTC cell migration and invasion. I. Apoptosis of four groups on PTC cells was examined with flow cytometry analysis. *P < 0.05, **P < 0.01