Fig. 3
From: LncRNA CALML3-AS1 suppresses papillary thyroid cancer progression via sponging miR-20a-5p/RBM38 axis
miR-20a-5p is a target of CALML3-AS1. A. The binding sites between wild type CALML3-AS1 or mutated CALML3-AS1 and miR-20a-5p were predicted using LncTar analysis. Luciferase reporter assay was carried out in BCPAP and K1 cells to confirm the combination between CALML3-AS1 and miR-20a-5p. B. RNA pull-down assays were used to determine the interaction between CALML3-AS1 and miR-20a-5p. C. Relative miR-20a-5p expression while CALML3-AS1 overexpression in BCPAP and K1 cells. D. The relative miR-20a-5p expression was tested in both BCPAP and K1 cells using qRT-PCR. E. Pearson correlation analysis between miR-20a-5p and CALML3-AS1 expressions in 52 cases of PTC tissues. *P < 0.05, **P < 0.01