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Table 1 Advantages and disadvantages of OSNA

From: Application of One-Step Nucleic Acid Amplification (OSNA) in different cancer entities and usefulness in prostate cancer: a systematic review

Advantages Disadvantages
Fast, objective, automated, and reproducible technique Not valid for non-CK19 expressing tumours
Intraoperative analysis Trained pathologist needed (thorough dissection)
Analysis of the whole LN Potential contamination of the sample
Quantitative analysis:
• Cut-off points for macro and micrometastases
• TTL: potential predictive and prognostic factor
Not applicable in case of coexisting neoplasms with the same LN drainage
Ability to a more accurate identification of micrometastases No tissue left to re-analysis (except RNA-based molecular tests)