Fig. 5

Correlation analysis of the signature scores with the CMS1-4 subtypes. (a) The CMS classification system was used to classify 2250 CRC tumors: CMS1 (n = 305), CMS2 (n = 675), CMS3 (n = 347) and CMS4 (n = 685) as well as CMS-NA (n = 238) (that are not applicable to any single CMS1-4 subtype). The Kaplan–Meier (KM) survival analysis shows that the CMS1 and CMS4 tumors (vs CMS2 and CMS3 tumors) were significantly associated with poor overall survival (OS). (b) Spearman correlation heatmap of the signature scores with CMS1*, CMS2*, CMS3* and CMS4* scores (measuring a propensity of a tumor to fall into CMS1, CMS2, CMS2 and CMS4 classes, respectively) in 2250 CRC tumors. 13-gene BP –- 13-gene MEKi bypass resistance; 5-gene Dasa-S –- 5-gene dasatinib sensitivity; 18-gene MEK –- 18-gene MEK pathway activation. (c-g) Comparison of the signature scores among the CMS1-4 subtypes (n = 2012). Bars represent Mean with standard errors (SEM). P values are for two-tailed Mann Whitney test. (h) Scatter plots of Hu-Lgr5-ISC, Hu-EphB2-ISC, Hu-Late TA, and Hu-Proliferation vs EMT signature scores. The CMS1-4 subtypes are indicated by red (CMS4) vs orange (CMS3) vs green (CMS2) vs blue (CMS1) colors