Fig. 3

SRC activation was highly correlated with EMT and its associated genes and was strongly associated with regional metastasis and disease recurrence. Spearman correlation analyses in 2250 CRC tumors: (a) PC1 vs EMT signature scores and (b-d) SRC activation vs EMT, PC1, and CDH1 (an epithelial marker), VIM (a mesenchymal marker), as well as EMT-genes SMAIL2, TWIST1, TWIST2, ZEB1, and ZEB2, respectively. Signature cores were standardized by subtracting the score median and dividing by the score IQR (interquartile range). The gene expression levels of individual EMT-related genes were also similarly standardized to give relative gene expression levels (median expression was set to 0). Spearman correlation heatmaps in (e) 1485 primary tumors and (f) 764 metastatic tumors. Comparison of (g) SRC activation, (h) EMT, and (i) PC1 signature scores as well as (j) TWIST1 gene among 2202 metastatic (regional, distant) vs primary tumors (recurrent, other). Note that among 2250 tumors, 48 tumors without approximate data were excluded from analysis. Bars represent Mean with standard errors (SEM). P values are for two-tailed Mann Whitney test