Fig. 3

Circ_0064288 functions as ceRNA and sponges miR-335-5p. A. Bioinformatics databases (StarBase and Circular RNA Interactome) were used to predict the binding site of circ_0064288 to miR-335-5p. B. qRT-PCR was employed to detect circ_0064288 expression in the cytoplasm and nucleus of Huh7 and Hep3B cells. C. Dual-luciferase reporter gene experiment showed that the predicted binding sequence between circ_0064288 and miR-335-5p was functional. D. qRT-PCR was employed to detect miR-335-5p expression in HCC tissues and paracancerous tissues. E. qRT-PCR was used to detect miR-335-5p expression in normal hepatic epithelial cell line (THLE-3) and HCC cell lines (Huh7, Hep3B, HCCLM3, and MHCC97-L). F. The correlation between circ_0064288 expression and miR-335-5p expression in HCC tissues was evaluated by Pearson’s correlation coefficient. ***P < 0.001, ns, means no statistical significance. ***P < 0.001, ns, means no statistical significance