Fig. 1

Expression levels of B7-H3 and B7-H4 PAAD tissues. A Representative microphotographs revealing low B7-H3 expression in para-tumor tissues and low, medium, and high B7-H3 expression in tumor tissues using IHC staining. Brown, B7-H3. Blue, haematoxylin. Bar = 200 μm. B7-H3 was mostly localized to the cytomembrane of tumor cells and tumor stroma. B The semi-quantitative analysis of B7-H3 in tumor and para-tumor tissues. B7-H3 was significantly up-regulated in tumor tissues compared with para-tumor tissues. C Representative microphotographs revealing low B7-H4 expression in para-tumor tissues and low, medium, and high B7-H4 expression in tumor tissues using IHC staining. Brown, B7-H4. Blue, haematoxylin. Bar = 200 μm. B7-H4 was mostly localized to the cytomembrane of tumor cells but not tumor stroma D The semi-quantitative analysis of B7-H4 in tumor and para-tumor tissues. B7-H4 was significantly up-regulated in tumor tissues compared with para-tumor tissues. E Correlation between B7-H3 and B7-H4 expression in the TMA cohort. No obvious correlation was found between B7-H3 and B7-H4 expression. F Correlation between B7-H3 and B7-H4 mRNA expression in the TCGA database. B7-H3 was positively correlated with B7-H4 expression