Fig. 5

Secreted TN-C induces activation of NF-κB signaling, leading to EMT. A Western blot indicates that secreted TN-C induces upregulation of N-Cad, Vim, Snail, MMP₂ and MMP₉ and downregulation of E-Cad; GAPDH was a loading control; B Real time PCR agrees with Western blot, *P < 0.05, ^△P > 0.05; C Immunofluorescent staining suggests that secreted TN-C contributes to nuclear translocation of NF-κB (P65), activating signaling, Bar: 20 μm