Fig. 4

TN-C promotes tumor invasion, migration and proliferation as a secreted glycoprotein. A Boyden chamber assay indicates that TN-C promotes BCa cell migration and invasion as secreted glycoprotein. Left: cartoon of tumor cell with or without Matrigel (pink area); Middle: representative figures, Bar: 100 μm; Right: quantification, P < 0.05. The exogenous TN-C (Ex) promotes tumor cell migration and invasion (T24^{si TN-C}-Ex Vs T24^{si TN-C}-con, 5637^Vec-Ex Vs 5637^Vec-con), whereas the neutralizing antibody of TN-C (Anti) attenuates this phenomenon (T24^sc-Anti Vs T24^sc-con, 5637^TN-C-Anti Vs 5637^TN-C-con); ^*P < 0.05, ^△P > 0.05; B BrdU incorporation suggests that TN-C promotes BCa cell proliferation as secreted glycoprotein. Upper: representative figures, Bar: 100 μm; Lower: relative fluorescent intensity. The exogenous TN-C (Ex) leads to tumor cell proliferation (T24^{si TN-C}-Ex Vs T24^{si TN-C}-con, 5637^Vec-Ex Vs 5637^Vec-con), and TN-C neutralizing antibody inhibits this (T24^sc-Anti Vs T24^sc-con, 5637^TN-C-Anti Vs 5637^TN-C-con); C Wound healing analysis indicates that TN-C promotes BCa cell wound healing also as secreted glycoprotein. Ex Vs con (T24^{si TN-C}-Ex Vs T24^{si TN-C}-con, 5637^Vec-Ex Vs 5637^Vec-con) indicates that exogenous TN-C reduces wound-healing time, and Anti Vs con (T24^sc-Anti Vs T24^sc-con, 5637^TN-C-Anti Vs 5637^TN-C-con) indicates that neutralizing antibody prolongs this duration. Bar: 100 μm