Fig. 3

Gene expression analysis of REST knockdown in MCF-7 cells. Gene expression analysis using RT-qPCR showing siRNA knockdown of REST in MCF-7 cells treated with control (PBS), estradiol (10 nM), progesterone (1uM), or both leads to differences in overexpression of REST targets. Error bars indicate ± SD. Student’s t-test was performed, *P < 0.05,**P < 0.01