Fig. 8

Detection of HPV encoded transcripts in cervical cancer exosomes. A Schematic representation of HPV16 and HPV18 genomes and the primer coordinates for targeted amplification of E6 and E7 transcripts. Primers are marked as solid arrows with their nucleotide binding position, their corresponding sequences and amplicon size. The size of amplicons from full length transcripts and spliced variants are indicated in respective panels (in red font). B Representative agarose gel photographs of specific PCR amplicons of HPV16/18 E6 and E7 in SiHa and HeLa cells and in their respective exosomal RNA. RT-PCR reaction performed on cDNA prepared from (2 μg/10 μl reaction) of genomic or exosomal RNA. Exo.- exosomal cDNA, M-marker, PD-primer dimer