Fig. 1

OGR1 upregulates CSNK2A3 (CK2α intronless gene) and NEP but not CSNK2A1 in A549: A A549 cells were transfected with pcDNA3.1-OGR1 (OGR1) or pcDNA3.1 (Vector), transcript expressions of CSNK2A1, CSNK2A3, and NEP were analyzed in the semi-quantitative RT-PCR. β-actin was used as a control for equal loading. B DNA bands intensities for CSNK2A1, CSNK2A3, NEP were analyzed by using ImageJ software and represented graphically after normalization to actin. C Protein expressions of NEP and CK2α were assayed by Western blotting with specific antibodies and β-actin was used as a control for equal loading, D protein band intensities were analyzed by using ImageJ software, and represented graphically after normalization to actin. E Transcript expression of CSNK2A1 and CSNK2A3 were analyzed after OGR1 knockdown in A549 cells by expressing shRNAs against OGR1, cells expressing scrambled shRNA as control. F DNA band intensities for CSNK2A1 and CSNK2A3 were analyzed by using ImageJ software and represented graphically. The display of cropped gels and blots are to improve the clarity and conciseness of the presentation. However, full length gels and blots are also included in supplementary file 2. Replicate of NEP and actin blots are also included in supplementary file 2. Error bars represent the Mean ± SD of three independent experiments. The p-value of statistical significance was set as p < 0.05 (*)