Fig. 4

Hsa_circ_0000277 acted as a sponge for miR-873-5p in ESCC cells. A RIP assay was administrated to study whether hsa_circ_0000277 could bind to Ago2 protein. B Venn Diagram analysis was used for selecting the common miRNAs from circBank and circinteractome. C, D Pull-down assay with biotinylated hsa_circ_0000277 probe was implemented to explore which miRNA was captured by hsa_circ_0000277. E The binding sites between hsa_circ_0000277 and miR-873-5p in circinteractome and the presentation of the mutated sites. F The overexpression effect of miR-873-5p mimic on miR-873-5p was evaluated by RT-qPCR. G Dual-luciferase reporter assay was performed for affirming that hsa_circ_0000277 could interact with miR-873-5p. H The RT-qPCR was employed to determine miR-873-5p level after transfection of sh-NC or sh-circ_0000277#1. *P < 0.05