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Fig. 1 | BMC Cancer

Fig. 1

From: Circular RNA hsa_circ_0000277 promotes tumor progression and DDP resistance in esophageal squamous cell carcinoma

Fig. 1

Hsa_circ_0000277 was upregulated in ESCC cells and its characteristics as a circRNA. A The back-splicing information of hsa_circ_0000277 and Sanger sequencing in the splice junction. B The detection of hsa_circ_0000277 was administrated exploiting RT-qPCR in ESCC (EC9706 and KYSE30) cells and control HET-1A cells. C, D The levels of hsa_circ_0000277 and its linear form (PDE3B mRNA) were assayed by RT-qPCR in EC9706 and KYSE30 cells treated with actinomycin D. E The RT-qPCR was used for analyzing hsa_circ_0000277 and PDE3B mRNA after RNA was exposed to RNase R. F Expression levels of hsa_circ_0000277, GAPDH and U6 were measured using RT-qPCR in the cytoplasmic or nuclear fraction. *P < 0.05

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