Fig. 2

Flow cytometry analysis for TAMs in cervical cancer tissue. Fresh biopsies of cervical cancer tissue were minced and stained immediately for phenotype analysis by flow cytometry. A, representative example of the gating strategy for macrophages isolated from viable CD45⁺ mononuclear cells of cervical cancer patients. Representative scatter diagrams (B) and histograms (C) of CD68⁺CD163⁺ TAMs in cervical cancer tissue before and after radiotherapy. Representative scatter diagrams (D) and histograms (E) of CD68⁺CCR7⁺ TAMs in cervical cancer tissue before and after radiotherapy. P value was calculated by Wilcoxon matched-pairs signed rank test