Fig. 7
RBM3 silencing halts cell cycle progression in T24 bladder cancer cells. Following collection of data for 2 × 104 cells, the cell population was gated and doublet discrimination was performed to identify single cells. Cell cycle analysis with the Watson Pragmatic algorithm was applied using FLowJo v10.6.1. on the single cell population for each sample. a In RT4 bladder cancer cells, no effect of RBM3 silencing on cell cycle distribution was observed, as detected by flow cytometry following propidium iodide staining. b In T24 cells, RBM3 silencing resulted in an increase in G1-phase and a significant decrease in S-phase. c Relative mRNA levels of cell cycle checkpoint markers in RT4 and T24 cells transfected with siRNA targeting RBM3 compared to non-targeting siRNA control (dashed line). Mean ± SEM from at least four independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001, two-tailed unpaired t test. d A schematic overview of the potential role of RBM3 in cell cycle progression based on findings from RNA-sequencing, cell cycle analysis as well as measurement of mRNA levels of selected cell cycle markers (red outline) after siRBM3 transfection in T24 cells