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Fig. 3 | BMC Cancer

Fig. 3

From: Pre-clinical and clinical studies on the role of RBM3 in muscle-invasive bladder cancer: longitudinal expression, transcriptome-level effects and modulation of chemosensitivity

Fig. 3

Decreased RBM3 levels after siRNA transfection. a qRT-PCR analysis of relative RBM3 mRNA expression levels in RT4 and T24 cells normalized to control (18S). b Visualization of RBM3 protein expression in bladder cancer cell lines RT4 and T24 after siRNA-mediated RBM3 knockdown compared to non-targeting siRNA control. Representative images taken with cellSens Dimension software at 20X magnification. Scale bar represents 20 μm. c Reduced RBM3 mRNA levels after siRNA transfection compared to non-targeting siRNA control measured by qRT-PCR. d RBM3 protein expression levels after siRNA transfection compared to non-targeting siRNA control detected via Western blot. Quantification of relative protein expression, normalized to total protein content, was performed using Empiria Studio Software of samples derived from the same experiment and with blots processed in parallel. Images shown represent one of three independent experiments and are cropped from full-length blots presented in Additional file 4: Fig. S2. Bars represent mean ± SEM from at least three independent experiments performed in triplicate. **p < 0.01, ***p < 0.001, two-tailed unpaired t test

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BMC Cancer

ISSN: 1471-2407

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