Fig. 7
From: Serotoninergic receptor ligands improve Tamoxifen effectiveness on breast cancer cells
Effect of low doses of SER79 and SER68 on MCF7 and MCF10A cells. a Estrogen-starved MCF7 cells (48 h) were treated with Tamoxifen (5 μM) in presence of E2 (100nM) and SER79 (5 μM) or SER68 (20 μM, 5 μM). As positive control, the cells were treated with E2 and SER alone (without Tamoxifen, dotted line). After 72 h, cell viability was assessed by sulforhodamine B assay (see Methods). The results were reported as percentage of viable cells compared to positive control, considered as maximum viability (100%). b Estrogen-starved MCF7 cells (48 h) were treated with SER79 (5 μM) or SER68 (20 μM) in presence of E2 (100 nM). After 72 h, mRNA levels of CTGF were determined by qPCR (see Methods and Table 1). Data were normalized on Ribosomal Protein S23 (Rps23) gene as internal standard. Bars represent CTGF mRNA levels in MCF7 treated with SER relative to those in untreated cells (dotted line). c MCF10A non-cancer breast epithelial cells were treated with SER79 (5 μM) or SER68 (20 μM). After 72 h, cell viability was assessed by sulforhodamine B assay (see Methods). The results were reported as percentage of viable cells compared to untreated cells (dotted line), considered as maximum viability (100%). a-c Data represent the mean ± SD of at least three independent triplicate experiments. * denotes statistically significant values compared with (a) positive control (*adjp<0.05,**adjp<0.01), (b, c) untreated cells (*pval<0.05, *pval<0.01); # denotes statistically significant values compared with cells treated with Tamoxifen in absence of SER (#pval<0.01)