Fig. 4
125I seed activated miR-615 expression by inducing demethylation of miR-615 promoter. A miR-615 expression of cells in each group was detected using RT-qPCR. B,C The methylation level of miR-615 promoter was detected using MSP. D,E The enrichment of DNMT1, DNMT3a and DNMT3b in miR-615 promoter region was measured using ChIP, and IgG acted as the negative control. F,G The methylation level of miR-615 promoter was detected using meDIP. The cell experiments were repeated 3 times independently. Data are presented as mean ± standard deviation. Data in panels A/F/G were analyzed using t test, and data in panels D/E were analyzed using one-way ANOVA, followed by Tukey’s multiple comparisons test. *p < 0.05 vs. the control or DMSO groups