Fig. 2

Downregulation of circN4BP2L2 promoted epithelial ovarian cancer cell migration and invasion in vitro. a Relative expression level of circN4BP2L2 in 5 different EOC cell lines (SKOV3, OVCAR3, CAOV3, HO8910 and TOV-112D) and one normal ovarian epithelial cell line (IOSE80); b After circN4BP2L2 RNA interference, the morphology of tumor cells in interference group (N1) significantly changed into spindle shape compared to that in control group (NC); c CircN4BP2L2 expression level did not affect tumor cell proliferation as indicated by CCk-8 assays in SKOV3 cells. Data are mean ± standard deviation from triplicate experiments (P > 0.05, Student’s t-test). d Transwell migration assay was measured and the results showed that downregulation of circN4BP2L2 promoted tumor cell migration (*P < 0.05, **P < 0.01, Student’s t-test); e Transwell invasion assay was measured and the results showed that downregulation of circN4BP2L2 promoted tumor cell invasion (*P < 0.05, **P < 0.01, Student’s t-test); f colony formation assays were performed and the results from triplicate experiments demonstrated that circN4BP2L2 did not affect the colony formation of SKOV3 cells (*P < 0.05, **P < 0.01, Student’s t-test); g Wound healing assay showed that downregulation of circN4BP2L2 resulted in a faster closing of scratch wounds (*P < 0.05, **P < 0.01, Student’s t-test); h and i CircN4BP2L2 expression level did not affect tumor cell cycle or apoptosis