Fig. 5

Overexpression TRAP1 expression in CAFs suppress tumor growth in vivo. A TRAP1 overexpression was confirmed using RT-PCR and Western blotting. B experimental timeline: HSC3 cells (1.0 × 106 cells/mouse) and conditioned CAF (1.0 × 106 cells/mouse) as indicated were inoculated to Balb/C-nu mice subcutaneously. C Tumor growth curves in control and experiment groups were measured as tumor volume over time. D Differences in tumor volume between control and treatment groups were analyzed statistically as described in the methods. *p < 0.05. p values calculated using two-way ANOVA with Tukey for multiple comparison. E Mice were sacrificed at day 22 after tumor cell injection. Photographs of tumor in nude mice. Differences in tumor volume and weight between control and treatment groups were determined by two-way ANOVA with p < 0.05 as being statistically significant. F Western blotting and Immunohistochemistry were employed to examine the expression levels of TRAP1 in four in vivo xenograft models