Fig. 4

TRAP1 regulated the metabolism of CAFs by reducing OXPHOS. A TRAP1-GFP CAFs were verified with fluorescence microscopy; Efficiency of TRAP1 overexpression was confirmed using Western blotting. B TRAP1 overexpression CAFs and control cells seeded in growth medium were enumerated at 24 h, 48 h, 72 h and 96 h. C Basal OCR, ATP production, maximal respiration and SRC were assessed in CAFs after TRAP1 overexpression and vector transfected cells were used as controls. Data are presented as mean ± SD of three independent experiments, and n.s represents no significant difference. Statistically significant relationships are indicated by *p < 0.05, ***p < 0.005 and ****p < 0.0001