Fig. 2

CAFs demonstrate stronger mitochondrial respiratory capacity than NFs. A Metabolism capacity of four patients derived CAFs and NFs were assessed by Seahorse flux analyzer. Data are representative of four independent experiments. B-C O2 consumption rates (OCR) and extracellular acidification rate (ECAR) were measured in real time under basal conditions. Basal OCR, *p < 0.05 (B) and OCR/ECR ratio **p < 0.01 (C)for CAFs versus NFs. Data are representative of four independent experiments. D After FCCP injection, Maximal Respiration of CAFs and NFs were assessed. **p < 0.01 for CAFs versus NFs. Data are representative of four independent experiments. E ATP production of CAFs and NFs were measured by Seahorse flux analyzer. ***p < 0.005 for CAFs versus NFs. Data are representative of four independent experiments. F Spare respiratory capacity (maximal respiration minus basal respiration) was assessed by Seahorse flux analyzer, *p < 0.05 for CAFs versus NFs. Data are representative of four independent experiments. G Confocal images show CAFs and NFs stained with Mitotracker (green) and nucleu (blue); scale bars represent 50 μm. H Western blotting was employed to examine the expression levels of PGC-1α and TFAM in CAF and NFs. Data are presented as mean values ± SD of three independent experiments, and n.s represents no significant difference. Statistically significant relationships are indicated by *p < 0.05