Fig. 3

IL8 overexpression promoted HN6 migration, invasion, tumor formation, and spheroid formation. The transfection efficiency is shown in the microscope image (A). IL8 expression was verified by real-time PCR (B), Western Blot (C), and ELISA (D). Transwell assay was used to explore the migration and invasion ability between HN6 Oe and HN6 Mock (E). Tumor formation was examined by xenograft animal model (F). A spheroid formation assay was used to observe the spheroid formation when treated with IL8 (G).