Fig. 4

Exosomes derived from sensitive GC cells were taken up by SGC-7901/5-FU cells. a Confocal microscopy showed exosome internalization by SGC-7901/5-FU recipient cells after co-incubation with PKH26-labeled SGC-7901 exosomes. Red: exosomes stained with PKH26, blue: DAPI. Scale Bars = 20 μm. b The MGC-803-pLVX stable cells were placed in the upper chamber and coincubated with SGC-7901/5-FU cells seeded in the lower chamber in a coculture system with a 0.4 μm pore membrane. Green fluorescence was observed in the SGC-7901/5-FU recipient cells under the fluorescence microscope. Scale Bars = 35 μm. c The percentage of green fluorescence signals in SGC-7901/5-FU resistant GC cells were determined by flow cytometry. Data were expressed as the mean ± SD (n = 3). Statistical significances were determined using one-way ANOVA followed by Dunnett’s test. ^**P < 0.01, compared with the group of MGC-803 cells